Alice was a leading party in the conflict against the Umbrella Corporation during the Global T-virus Pandemic. Originally a high-ranking Umbrella security operative, she joined a deep cover operation by members of the US law enforcement to reveal the company's illegal viral research data to the world. This plan ultimately failed when her co-worker, Spence stole the virus samples with the intention of getting rich on the black market and leaked the virus into the lab to kill the research staff in the confusion. Both Alice and Spence were knocked unconscious when the security system released noxious gas and were rendered amnesiac.
Infected after escaping the Zombie-infested facility, Alice was taken away by Umbrella for testing. Enhanced by a T-virus infection; cured and re-infected, Alice became the test subject of an Umbrella research project designated "Project Alice"; granted with superhuman abilities, she was able to survive the viral outbreak in Raccoon City and several years of the T-virus' spread across the Earth. As she had not succumbed to the T-virus and transformed into a Zombie, the acquisition of Alice became a major goal to the company as a means of surviving the pandemic. Alice ultimately crippled Umbrella's leadership nonetheless, paving the way for its Red Queen computer system to take full control of its remaining assets, now intent on humanity's destruction. Unknown to Alice, this was another Umbrella ploy to wipe out humanity, a ploy that was highly successful. After exposure to the T-Virus, Alice's abilities became enhanced. Following the fall of Washington, DC, Alice was contacted by the Red Queen to bring a final end to the Global T-Virus pandemic and Umbrella. With the help of Claire Redfield, Alice returned to the Hive where she discovered her true nature as a clone of Alicia Marcus. With the aid of the Red Queen, Claire and Alicia, Alice destroyed the Hive and Umbrella and unleashed an anti-virus capable of bringing an end to the global pandemic.